Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mesomycoplasma mobile |
Protein Variants | Comment | Organism |
---|---|---|
K598A | the mutation simultaneously reduces the tRNA-binding strength and aminoacylation and editing capacities of the enzyme's leucine-specific domain | Mesomycoplasma mobile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
tRNALeu(GAG) | in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C | Mesomycoplasma mobile | |
0.0076 | - |
tRNALeu(UAG) | in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C | Mesomycoplasma mobile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesomycoplasma mobile | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mesomycoplasma mobile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu(GAG) | - |
Mesomycoplasma mobile | AMP + diphosphate + L-leucyl-tRNALeu(GAG) | - |
? | |
ATP + L-leucine + tRNALeu(UAG) | - |
Mesomycoplasma mobile | AMP + diphosphate + L-leucyl-tRNALeu(UAG) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Mesomycoplasma mobile |
LeuRS | - |
Mesomycoplasma mobile |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
tRNALeu(GAG) | in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C | Mesomycoplasma mobile | |
2 | - |
tRNALeu(UAG) | in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C | Mesomycoplasma mobile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mesomycoplasma mobile |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
tRNALeu(GAG) | in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C | Mesomycoplasma mobile | |
260 | - |
tRNALeu(UAG) | in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C | Mesomycoplasma mobile |